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eDNA Barcoding

How to detect the presence of a species from an environmental sample?

VigiDNA S technologies, based on the “eDNA Barcoding” approach, are mainly used for the monitoring of rare or secretive species in aquatic or terrestrial environments. Those analyses can be performed from water, soil or faecal samples.

Detection of a target species from a water sample

The “eDNA Barcoding” approach for the monitoring of aquatic species has been developed in 2008 by the Laboratoire d’Ecologie Alpine. It is based on the extraction of DNA present in a water sample and its amplification using a specific primer pair for the target species. Scientific studies lead by SPYGEN and its partners have demonstrated that this new method enables improved detection of rare or elusive aquatic species (the detectability depends on the target species) and a reduction  in survey costs. It also enables to avoid any risk of introduction of pathogens or invasive species during sampling.

For more information, you can download: Dejean et al. 2012.pdf; Biggs et al. 2015.pdf

Target species identification from faeces

This expertise was developed in 2011 by SPYGEN and the Laboratoire d’Ecologie Alpine for the identification of mammal species. It is based on the use of a “”mammal”“ universal primer pair and Sanger sequencing technologies. This non-invasive method is mainly used for the monitoring of micromammal species that are difficult to observe or identify using traditional field methods.

*Patented technology (CNRS – Université Grenoble 1) 

Analyses protocols