We compared SPYGEN’s standard protocol with a simulated result of a basic protocol. In marine waters, 40% of species went undetected with the basic protocol, while in freshwater, 33% of species were missed.
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eDNA technology offers a non-invasive, replicable, and observer-independent method for monitoring biodiversity. However, “eDNA” now refers to a wide range of protocols that vary greatly in sophistication – raising a critical question: are they all equally effective?
To explore this, we compared SPYGEN’s standard protocol for marine and freshwater environments with a simulated result of a basic protocol at two real-world sites.
- Marine site: 40% of species went undetected and 100% of threatened species were missed with the basic protocol.
- Freshwater site: 33% of species went undetected and 67% of threatened species were missed with the basic protocol.