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Since 2007, scientists at SPYGEN actively participate to the development of biodiversity monitoring methods based on eDNA and are co-authors of numerous publications in this field.


Pont, D., Rocle, M., Valentini, A., Civade, R., Jean, P., Maire, A., Roset, N., Schabuss, M., Zornig, H., Dejean, T. 2018. Environmental DNA reveals quantitative patterns of fish biodiversity in large rivers despite its downstream transportation. SCI Reports, DOI: 10.1038/s41598-018-28424-8 (PDF)


Guillerault, N. , Bouletreau, S. , Iribar, A. , Valentini, A. and Santoul, F. 2017. Application of DNA metabarcoding on faeces to identify European catfish Silurus glanis diet. J Fish Biol, 90: 2214-2219. doi:10.1111/jfb.13294 (PDF)

Lopes, C. M., Sasso, T. , Valentini, A. , Dejean, T. , Martins, M. , Zamudio, K. R. and Haddad, C. F. 2017. eDNA metabarcoding: a promising method for anuran surveys in highly diverse tropical forests. Mol Ecol Resour, 17: 904-914. doi:10.1111/1755-0998.12643 (PDF)

Sasso, T., Lopes, C.M., Valentini, A., Dejean, T., Zamudio, K.R., Haddad, C.F.B, Martins, M. 2017. Environmental DNA characterization of amphibian communities in the Brazilian Atlantic forest: Potential application for conservation of a rich and threatened fauna. Biological Conservation, 215, 225-232. ISSN 0006-3207. (PDF)


Leese, F., …, Valentini, A., et al. 2017. DNAqua-Net: Developing new genetic tools for bioassessment and monitoring of aquatic ecosystems in Europe. Res Idea outcomes, DOI: 10.3897/rio.2.e11321 (PDF)

Bellemain, E., Patricio, H., Gray, T., Guegan, F., Valentini, A., Miaud, C., Dejean, T. 2016. Trails of river monsters: Detecting critically endangered Mekong giant catfish Pangasianodon gigas using environmental DNA. Global Ecology and Conservation 7, 148–156 (PDF).

Civade, R, Dejean, T., Valentini, A., Roset, N., Raymond, J-C., Bonin, A., Taberlet, P., Pont, D. 2016. Spatial Representativeness of Environmental DNA Metabarcoding Signal for Fish Biodiversity Assessment in a Natural Freshwater System. PLoS ONE 11(6): e0157366. (PDF)

Miaud, C., Dejean, T., Savard, K., Millery-Vigues, A., Valentini, A., Curt Grand Gaudin, N., Garner, T.W.J. 2016. Invasive North American bullfrogs transmit lethal fungus Batrachochytrium dendrobatidis infections to native amphibian host species. Biological Invasions, DOI 10.1007/s10530-016-1161-y.

Elbrecht, V., Taberlet, P., Dejean, T., Valentini, A., Usseglio-Polatera, P., Beisel, J-N., Coissac, E., Boyer, F., Leese, F. 2016. Testing the potential of a ribosomal 16S marker for DNA metabarcoding of insects. PeerJ 4:e1966; DOI 10.7717/peerj.1966. (PDF)

Valentini, A., Taberlet, P., Miaud, C., Civade,  R., Herder, J., Thomsen, P. , Bellemain, E., Besnard, A.,  Croissac, E., Boyer, F., Gaboriaud, C., Jean, P., Poulet, N., Roset, N., Copp, G., Geniez, P., Pont, D., Argillier, C., Baudoin, J.-M., Peroux, T., Crivelli, A., Olivier, A., Acqueberge, M., Le Brun, M., Møller, P. R., Willerslev, E., Dejean, T. 2016. Next-generation monitoring of aquatic biodiversity using environmental DNA metabarcoding. Molecular Ecology 25, 929-942. (PDF)

Secondi, J., Dejean, T., Valentini, A., Audebaud, B., Miaud, C. 2016. Detection of a global aquatic invasive amphibian, , using environmental DNA. Amphibia-Reptilia, 37, 131-136, DOI:https://doi.org/10.1163/15685381-00003036 (PDF)

Schneider, J., Valentini, A., Dejean, T., Montarsi, F., Taberlet, P., Glaizot, O., et al. 2016. Detection of Invasive Mosquito Vectors Using Environmental DNA (eDNA) from Water Samples. PLoS ONE 11(9): e0162493. https://doi.org/10.1371/journal.pone.0162493 (PDF)


Biggs, J., Ewald, N., Valentini, A., Gaboriaud, C., Dejean, T., Griffiths, R.A., Foster, J., Wilkinson, J.W., Arnell, A., Brotherton, P., Williams, P., Dunn, F. 2015. Using eDNA to develop a national citizen science-based monitoring programme for the great crested newt. Special Issue: Environmental DNA: A powerful new tool for biological conservation, Biological Conservation 183, 19-28. (PDF)


Tréguier, A., Paillisson, J.M., Dejean, T., Valentini, A., Schlaepfer, M., Roussel, J.M. 2014. Environmental DNA surveillance for invertebrate species: advantages and technical limitations to detect invasive crayfish Procambarus clarkii in freshwater ponds. Journal of Applied Ecology 51(4), 871-879. (Abstract)

Giampaoli, S., Berti, A., Di Maggio, R.M., Pilli, E., Valentini, A., Valeriani, F., Gianfranceschi, G., Barni, F., Ripani, L., Romano Spica, V. 2014.  The environmental biological signature: NGS profiling for forensic comparison of soils. Forensic Science International 240:41-7. doi: 10.1016/j.forsciint.2014.02.028. (Abstract)

Herder, J.E., Valentini, A., Bellemain, E., Dejean, T., van Delft, J.J.C.W., Thomsen P.F., Taberlet, P., 2014. Environmental DNA – a review of the possible applications for the detection of (invasive) species. Stichting RAVON, Nijmegen. Report-104. (PDF)


Ibanez, S., Manneville, O., Miquel, C., Taberlet, P., Valentini, A., Aubert, S., Coissac, E., Colace, M.P., Duparc, Q., Lavorel, S., Moretti, M. 2013. Plant functional traits reveal the relative contribution of habitat and food preferences to the diet of grasshoppers. Oecologia 173(4), 1459-1470. (Abstract)


Dejean, T., Valentini, A., Miquel, C., Taberlet, P., Bellemain, E., Miaud, C. 2012. Improved detection of an alien invasive species through environmental DNA barcoding: the example of the American bullfrog Lithobates catesbeianus. Journal of Applied Ecology 49 (4), 953-960. (PDF)

Yoccoz, N.G., Brathen, K.A., Gielly, L., Haile, J., Edwards, M.E., Goslar, T., Von Stedingk, H., Brysting, A.K., Coissac, E., Pompanon, F., Sonstebo,  J.H., Miquel, C., Valentini, A., De Bello, F., Chave, J., Thuiller, W., Wincker, P., Cruaud, C., Gavory, F., Rasmussen, M. 2012. DNA from soil mirrors plant taxonomic and growth form diversity. Molecular Ecology 21(15), 3647-3656. (Abstract)


Dejean, T., Valentini, A., Duparc, A., Pellier-Cuit, S., Pompanon, F., Taberlet, P., Miaud, C. 2011. Persistence of Environmental DNA in Freshwater Ecosystems. PLoSOne 6 (8), 1-4. (PDF)

Kowalczyk, R., Taberlet, P., Coissac, E.,  Valentini, A., Miquel, C., Kaminski, T., Wojcik,  J.M. 2011. Influence of management practices on large herbivore diet—Case of European bison in Białowieża Primeval Forest. Forest Ecology and Management 261(4), 821-828. (Abstract)


Valentini, A., Miquel, C., Taberlet, P. 2010. DNA Barcoding for Honey Biodiversity. Diversity 2(4), 610-617. (PDF)


Pegard, A., Miquel, C., Valentini, A., Coissac, E., Bouvier, F., Francois, D., Taberlet, P., Engel, E., Pompanon, F., 2009. Universal DNA-Based Methods for Assessing the Diet of Grazing Livestock and Wildlife from Feces. Journal of Agricultural and Food Chemistry 57(13), 5700-5706. (Abstract)

Soininen, E.M., Valentini, A., Coissac, E., Miquel, C., Gielly, L., Brochmann, C., Brysting, A.K., Sonstebo, J.H., Ims, R.A., Yoccoz, N.G., Taberlet, P., 2009. Analysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures. Frontiers in Zoology 6(16) doi:10.1186/1742-9994-6-16. (PDF)

Valentini, A., Pompanon, F., Taberlet, P., 2009. DNA barcoding for ecologists. Trends in Ecology & Evolution  24(2), 110-117. (Abstract)

Valentini, A., Miquel, C., Nawaz, M.A., Bellemain, E., Coissac, E., Pompanon, F., Gielly, L., Cruaud, C., Nascetti, G., Winker, P., Swenson, J.E., Taberlet, P. 2009. New perspectives in diet analysis based on DNA barcoding and parallel pyrosequencing: the trnL approach. Molecular Ecology Resources 9(1), 51-60. (Abstract)


Bellemain, E., Nawaz, A., Valentini, A., Swenson, J.E, Taberlet, P. 2007. Genetic tracking of the brown bear in northern Pakistan and implications for conservation. Biological Conservation 134, 537-547. (Abstract)

Taberlet, P., Coissac, E., Pompanon, F., Gielly, L., Miquel, C., Valentini, A., Vermat, T., Corthier, G., Brochmann, C., Willerslev, E. 2007. Power and limitations of the chloroplast trnL (UAA) intron for plant DNA barcoding. Nucleic Acids Research 35(3) doi: 10.1093/nar/gkl938. (PDF)